Monica SCALI

BIOSKETCH AND CV

Monica Scali obtained her degree with honors in Biological Sciences from the University of Siena in 1991. In 1994 she did a CNR fellowship at the Dept of Molecular Plant Physiology, Radboud University, in Nijmegen (The Netherlands), getting familiar on higher plant reproduction and on the male side of the fertilization process using techniques ranging from genetics to molecular and cell biology. She joined the University of Siena in 1995 as staff scientist, returning to Dept. of Molecular Plant Physiology as visiting scientist in 1996. In 1995 she applied successfully for a Doctoral Research Fellowship at the University of Siena and in 1999 she obtained her Ph.D. degree in Environmental Sciences. At present, she is a Senior Scientist at the Department of Life Sciences.

 

Research Interests

Current research topics include:

Plant and animal characterization by genetic fingerprinting

Genomic analysis for food safety and traceability: Wine and meat molecular traceability

Reproductive biology of higher plants and plant proteomics

Technical expertise

DNA and RNA isolation, gene cloning, differential gene expression (microarrays, in situ hybridizations)

Plant Genetic transformation by Agrobacterium and particle bombardment (Gene gun)

Plant genotyping by SSR markers, including Mediterranean crops such as grapevine (Vitis vinifera L.)

Protein extraction. Separation of proteins through 1D- and 2D-electrophoresis.

 

Teaching Activities

Tutor activity in the Work Alternation Project of the Italian school.

Contract Professor for the training courses of the profession of biologist.

 

Selected Publications

Xiaolin Wu, Monica Scali, Claudia Faleri, Wei Wang. (2013) “Polyclonal antibody preparation and immunolocalization of maize (Zea mays) seed protein EMB564”. Plant Omics Journal, 6(5): 359-363. ISSN: 1836-3644.

Xiaolin Wu, Erhui Xiong, Wei Wang, Monica Scali, Mauro Cresti. (2014) “Universal sample preparation method integrating trichloroacetic acid/acetone precipitation with phenol extraction for crop proteomic analysis”. Nature Protocols, 9: 362–374. Doi:10.1038/nprot.2014.022.

Scai Monica, Paolucci Elisa, Bigliazzi Jacopo, Cresti Mauro, Rita Vignani. (2014). Vineyards genetic monitoring and Vernaccia di San Gimignano wine molecular fingerprinting. Advances in Bioscience and Biotechnology (ABB), 5: 142-154. DOI: 10.4236/abb.2014.52018.

Alessandra Moscatelli, Assunta Gagliardi, Lilly Maneta-Peyret, Luca Bini, Nadia Stroppa, Elisabetta Onelli, Claudia Landi, Monica Scali, Aurora Irene Idilli and Patrick Moreau. (2015). Characterization of detergent-insoluble membranes in pollen tubes of Nicotiana tabacum (L.). Biology Open, 4: 378-399. DOI: 10.1242/bio.201410249.

Monica Scali, Rita Vignani, Marco Quintetti, Jacopo Bigliazzi, Elisa Paolucci, Mauro Cresti. (2015) Genetic traceability of Cinta Senese pig (Sus scrofa domesticus L.): a study of the meat and processed products by microsatellite markers. Food and Nutrition Sciences, 6: 712-726.

Claudio Milanesi, Monica Scali, Rita Vignani, Franco Cambi, Lucas Dugerdil, Claudia Faleri, Mauro Cresti. (2016). Archaeobotanical reconstructions of vegetation and report of mummified apple seeds found in the cellar of a first century Roman villa on Elba Island, C. R. Biologies, http:// dx.doi.org/10.1016/j.crvi.2016.09.003

For the complete list of publications see at: