Luca Bini is Prof of Biochemistry and Proteome Sciences. He graduated in Biology before completing a PhD in Biotechnology. He spent some periods at the Geneva University (Switzerland) where he learned basic proteomic technologies. He is internationally well known as a contributor to the foundation of proteome science and he is the responsible of the Functional Proteomics lab. at the Siena University. Prof. Bini is one of the founders of the European Proteomics Association as well as of the Italian Proteomics Association and he is on the editorial boards of several leading proteomics journals.
- Proteomic analysis of clinical specimens such as cells, biopsies and body fluids in different patho-physiological conditions (breast, stomach and lung cancer, lung fibrosis, acute phase, cellular differentiation and signaling, apoptosis, etc..) to identify prognostic/diagnostic markers.
- Study of post-translational modifications (phosphorylation, oxidation, glycosilation) using 2D-immunoblotting and mass spectrometry.
- study of the immunproteome of infectious disease, as well as of the immuneresponse in autoantigenic neurodegenerative pathologies, such as Multiple Sclerosis, using patient sera.
- Proteomic characterization of different myopathies, fibrotic and oncologic diseases and in the molecular characterization of some genetic diseases such as Cornelia de Lange and Osteogenesis imperfecta.
- Study of the effects of different drugs in cells, tissue and body fluids.
Laura Bianchi, PhD.
Claudia landi, PhD.
Assunta Gagliardi, PhD student
Alessandro Armini, Technician
Currently, the main teaching activities of Prof. Bini are in the following courses:
Chimica Biologica (Biochemistry); Corso di Laurea in Scienze Biologiche, Università di Siena.
Scienza del Proteoma (Proteome Sciences); Corso di Laurea Magistrale in Biologia Molecolare e Cellulare, Università di Siena.
Gimigliano A., Mannini L., Bianchi L., Puglia M., Deardorff M.A., Menga S., Krantz I.D., Musio A., Bini L. Proteomic Profile Identifies Dysregulated Pathways in Cornelia de Lange Syndrome Cells With Distinct Mutations in SMC1A and SMC3 Genes. J. Proteome Res. 11, 6111-6123 (2012).
Bianchi L., Gagliardi A., Gioia R., Besio R., Tani C., Landi C., Cipriano M., Gimigliano A., Rossi A., Marini J.C., Forlino A., Bini L. Differential response to intracellular stress in the skin from osteogenesis imperfecta Brtl mice with lethal and non lethal phenotype: A proteomic approach. J. Proteomics 75, 4717-4733 (2012).
Archimandriti DT, Dalavanga YA, Cianti R, Bianchi L, Manda-Stachouli C, Armini A, Koukkou AI, Rottoli P, Constantopoulos SH, Bini L. Proteome Analysis of Bronchoalveolar Lavage in Individuals from Metsovo, Nonoccupationally Exposed to Asbestos. J. Proteome Res. 8, 860–869 (2009).
Bianchi L, Puglia M, Landi C, Matteoni S, Perini D, Armini A, Verani M, Trombetta C, Soldani P, Roncada P, Greppi G, Pallini V, Bini L. Solubilization methods and reference 2-DE map of cow milk fat globules. J. Proteomics 72, 853-864 (2009).
Lovato L., Cianti R., Gini B., Marconi S., Bianchi L., Armini A., Anghileri E., Locatelli F., Paoletti F., Franciotta D., Bini L., Bonetti B. Transketolase and CNPase I are specifically recognized by IgG autoantibodies in multiple sclerosis patients. Mol. Cell. Proteomics 7, 2337-2349 (2008).
Rottoli P., Magi B., Cianti R., Bargali E., Vagaggini C., Nikiforakis N., Pallini V., Bini L. Carbonylated proteins in bronchoalveolar lavage of patients with sarcoidosis, pulmonary fibrosis associated with systemic sclerosis and idiopathic pulmonary fibrosis. Proteomics 5, :2612-2618 (2005).
Bini L., Pacini S., Liberatori S., Valensin S., Pellegrini M., Raggiaschi R., Pallini V., Baldari C.T. Extensive temporally regulated reorganization of the lipid raft proteome following T-cell antigen receptor triggering. Biochem. J. 369, 301-309 (2003).
Zucchi I.*, Bini L.*, Albani D, Valaperta R, Liberatori S, Raggiaschi R, Montagna C, Susani L, Barbieri O, Pallini V, Vezzoni P, Dulbecco R. Dome formation in cell cultures as expression of an early stage of lactogenic differentiation of the mammary gland. Proc. Natl. Acad. Sci. U.S.A. 99, 8660-8665 (2002).
Zucchi I.*, Bini L.*, Valaperta R., Ginestra A., Albani D., Susani L., Sanchez J.-C., Liberatori S., Magi B., Raggiaschi R., Hochstrasser D.F., Pallini V., Vezzoni P., Dulbecco R. Proteomic dissection of dome formation in a mammary cell line: Role of tropomyosin-5b and maspin. Proc. Natl. Acad. Sci. U.S.A. 98, 5608-5613 (2001).
Bini L., Magi B., Marzocchi B., Arcuri F., Tripodi S., Cintorino M., Sanchez J.-C., Frutiger S., Hughes G., Pallini V., Hochstrasser D.F., Tosi P. Protein expression profiles in human breast ductal carcinoma and histologically normal tissue. Electrophoresis 18, 2832-2841 (1997).