Profiling and Functional Proteomics

Proteome is the entire protein set that may be produced by an organism. The spatiotemporal plasticity of proteome in different physiopathological states makes its investigation one of the most puzzling challenges in Life Sciences. Nonetheless, functional proteomics, by taking advantage of increasingly powerful bioinformatics resources, is rapidly giving new and more comprehensive insights into functions, interactions and pathways that the astonishing multiplicity of protein species act in biological systems.

The main research fields currently in progress are:

  • Proteomic analysis of clinical specimens in different physio-pathological conditions (breast, stomach and lung cancer, lung fibrosis, acute phase, cellular differentiation and signaling, apoptosis, etc..) to identify specific markers.
  • Characterization of post-translational modifications (phosphorylation, oxidation, glycosilation).
  • Study of the immunproteome of infectious diseases, as well as of the immuneresponse in autoimmune diseases.
  • Functional proteomic investigation on some rare diseases such as Osteogenesis imperfecta, Cystic fibrosis and Krabbe disease.
  • Study of drug effects in  culture cell lines, tissues and body fluids.
  • Determination of nanoparticle and microvesicle effects on different biological samples.

Researchers involved in the activities:

Luca BINI Full Professor https://docenti.unisi.it/it/bini-0

Laura BIANCHI (Assistant Professor) https://docenti.unisi.it/it/bianchi-3

Claudia LANDI (Post-Doc) https://www.researchgate.net/profile/Claudia_Landi2

National projects:

  1. “Endoplasmic Reticulum stress in osteogenesis imperfecta: novel potential therapeutic target“, Fondazione Cariplo - grant n. 2013-0612 to Luca Bini.
  2. "Targeting ER stress to treat osteogenesis imperfecta", Fondazione Telethon - grant GGP13098A to Laura Bianchi.

Both projects are developed in collaboration with Prof. Antonella Forlino - Dipartimento di Medicina Molecolare, Università di Pavia, Pavia; Prof. Jean-Charles Sanchez - University of Geneva, Department of Human Protein Sciences, Geneva, Switzerland; Prof Joan C. Marini - Bone and Extracellular Matrix Branch, NICHD, National Institute of Health, Bethesda, MD, USA.